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The Parthenon is a historic building in the city of Athens, Greece built on the orders of the Greek-Persian King Pericles to commemorate the. 480, after the arrival of the Athenians to the Parthenon, on July 14, 449, is executed by the Persians, thanks to the help of the.
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The Parthenon is a historic building in the city of Athens, Greece built on the orders of the Greek-Persian King Pericles to commemorate the. 480, after the arrival of the Athenians to the Parthenon, on July 14, 449, is executed by the Persians, thanks to the.
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We have succeeded in isolating and characterizing clones of Thermus thermophilus HB8 which exhibit high levels of 1,2-propanediol utilization and high resistance against hydroxyurea-induced cell-division inhibition. The generation times of these clones under aerobic growth conditions are significantly shorter than those of wild-type HB8. The enzymes required for 1,2-propanediol utilization, allozymes for glyoxalase I (Gor) and glyoxalase II (Gor II), were detected and purified to homogeneity by chromatographic procedures from crude extracts of all of these clones. The apparent molecular masses of Gor I and Gor II, which were determined by gel filtration and sucrose density gradient centrifugation, were about 76 and 60 kDa, respectively. The reactions of Gor I and Gor II with glyoxal gave a fluorescent product, glyoxal-2-ene-1-carboxylic acid, and the values of Km for these substrates were 0.40 and 0.44 mM, respectively. These results suggested that 1,2-propanediol is rapidly converted to glyoxal by Gor I and then to glyoxal-2-ene-1-carboxylic acid by Gor II. The overexpression of the allozyme of Gor I, Gor II, or both was found to confer resistance to hydroxyurea. The mechanism by which these allozymes confer resistance to cell-division inhibition was discussed.Skladištvo našega času: Mogoče javno iskala dokazi da je na određen način zaštićena znanost
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